ProteoSpin™ Abundant Serum Protein Depletion Kit

For the rapid depletion of abundant proteins from serum samples

  • Rapid and efficient removal of abundant proteins from serum and plasma samples
  • Process 10 samples in 30 minutes
  • Allows for visualization of low abundance proteins
  • Convenient and affordable spin column protocol
  • Generic protocol allows for depletion from human and other animal samples
Items Cat. # Size
ProteoSpin™ Abundant Serum Protein Depletion Kit 17300 25 preps

ProteoSpin™ Abundant Serum Protein Depletion Kit

This kit provides a fast and simple procedure for the effective depletion of major serum proteins including albumin, α-antitrypsin, transferrin and haptoglobin from serum and plasma samples. Such abundant proteins usually obscure less abundant proteins in gel electrophresis making them difficult to visualize and recover. The kit reduces sample complexity to allow for convenient investigation of less abundant proteins. In other applications such as mass spectroscopy, fractionating abundant proteins improves the resolution of less abundant proteins. The kit is 'generic' in that it is not antibody-based and can thus be used to deplete abundant proteins from any starting material - human or various other animals - making it more versatile, flexible and affordable than other kits. Resulting proteins are ready for a range of applications including 2D gel electrophoresis, SDS-PAGE, Mass Spectrometry, Protein microarrays, DIGE (Difference Gel Electrophoresis) and more.

Kit Specifications
Maximum Column Capacity
150 μg
Minimum Column Capacity
50 μg
Minimum Elution Volume
30 μL
Time to Process 10 Samples
30 minutes

Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C.

 

 

Kit Components
Component Cat. 17300 (25 preps)
Wash Solution G 125 mL
Elution Buffer C 8 mL
Protein Neutralizer 4 mL
Mini Spin Columns 25
Collection Tubes 25
Elution Tubes (1.7 mL) 25
Product Insert 1
Title Doublecortin-Like Kinase 1 Is Elevated Serologically in Pancreatic Ductal Adenocarcinoma and Widely Expressed on Circulating Tumor Cells
Journal PLoS ONE2015.
Authors Dongfeng Qu, Jeremy Johnson, Parthasarathy Chandrakesan, Nathaniel Weygant, Randal May, Nicole Aiello, Andrew Rhim, Lichao Zhao, Wei Zheng, Stanley Lightfoot, Shubham Pant, Jeremy Irvan, Russell Postier, James Hocker, Jay S. Hanas, Naushad Ali, Sripathi M. Sureban, Guangyu An, Michael J. Schlosser, Ben Stanger, Courtney W. Houchen
Title Identification of plasma Complement C3 as a potential biomarker for neuroblastoma using a quantitative proteomic approach.
Journal Journal of Proteomics2013.
Authors Kim PY, Tan O, Diakiw S, Carter D, Sekerye EO, Wasinger VC, Liu T, Kavallaris M, Norris MD, Haber M, Chesler L, Dolnikov A, Trahair TN, Cheung NK, Marshall GM, Cheung BB.
Title Quantitation of irbesartan and major proteins in human plasma by mass spectrometry with time-of-flight analyzer.
Journal Journal of Pharmaceutical and Biomedical Analysis2011.
Authors Lu CY, Feng CH.
Title Proteome analysis of rat serum proteins adsorbed onto synthetic octacalcium phosphate crystals.
Journal Analytical Biochemistry. 2010.
Authors Kanekoa H, Kamiieb J, Kawakamib H, Anadac T, Hondac Y, Shiraishid N, Kamakurae S, Terasakif T, Shimauchia H, Suzukic O.
Title Analysis of angiotensin II receptor antagonist and protein markers at microliter level plasma by LC?MS/MS
Journal Journal of Pharmaceutical and Biomedical Analysis.49 123?1282009.
Authors Lu CY, Chang YM, Tseng WL, Feng CH, Lu CY.