DNA Gel Extraction Kit

For rapid extraction of DNA from agarose gel fragments

  • Fast and easy recovery of DNA from agarose gel fragments
  • High recovery of desired DNA
  • Convenient spin column format
  • DNA is ready for ligation, restriction digestion, sequencing and more

Figure 2.  Efficient Recovery of Large DNA Fragments

Items Cat. # Size
DNA Gel Extraction Kit 13100 50 preps

DNA Gel Extraction Kit

This kit is designed for the rapid preparation and purification of DNA fragments that have been fractionated on agarose gels. The recovered DNA is free from agarose and other impurities, and is compatible with restriction enzyme digestion, ligation into vectors and sequencing. The protocol can be completed in 20 minutes.

Kit Specifications
Column Binding Capacity
10 μg
Maximum Weight of Gel Slice
400 mg
Average Recovery
70-90%
Size of DNA Purified
100 - 15,000 bp
Minimum Elution Volume
30 μL
Time to Complete 10 Purifications
30 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.

 

 

Kit Components
Component Cat. 13100 (50 preps)
Binding Buffer G 80 mL
Wash Solution A 12 mL
Elution Buffer B 8 mL
Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1
Title Expression analyses of some Beauveria bassiana genes in response to cuticles of four different insects
Journal Journal of Crop Protection. 2015.
Authors Masoumeh Kordi, Naser Farrokhi, Abolfazl Masoudi, Ali Derakhshan Shadmehri and Shahrokh Gharanjik
Title Host Exopolysaccharide Quantity and Composition Impact Erwinia amylovora Bacteriophage Pathogenesis.
Journal Applied and Environmental Microbiology2013.
Authors Roach DR, Sjaarda DR, Castle AJ, Svircev AM.
Title Natural occurrence of entomopathogenic nematode species (Rhabditida: Steinernematidae and Heterorhabditidae) in cotton fields of Tamil Nadu, India.
Journal Journal of Natural History2012.
Authors Seenivasana N, Prabhub S, Makeshc S, Sivakumara M.
Title Isolation and characterization of superior rumen bacteria of cattle (Bos taurus) and potential application in animal feedstuff.
Journal Open Journal of Animal Sciences. 2012.
Authors Krushna Chandra Das, Wensheng Qin.