Size-Select Kit for NGS Library Preparation

A rapid, simple and efficient procedure for the size selection and clean-up of fragmented DNA

  • Rapid and convenient column purification
  • Selection of DNA sizes of 200 bp - 500 bp for NGS
  • Provides high quality DNA fragments

Figure 1.  Efficient Purification of 200-500 bp DNA  Figure 2.  Efficient Purification of 200-500 bp DNA
      Efficient Purification of 200-500 bp DNA                 Efficient Purification of 200-500 bp DNA

Items Cat. # Size
Size-Select Kit for NGS Library Preparation

Size-Select Kit for NGS Library Preparation

This kit provides a rapid, simple and efficient procedure for the size selection and clean-up of fragmented DNA. The resulting DNA is suitable for use in next generation sequencing library preparation. The kit selectively isolates DNA ranging in size from approximately 200-500 bp from a mixture of fragmented DNA, and removes various impurities including un-incorporated adaptors and enzymes, without the use of magnetic beads or agarose gel extraction. The kit is robust and is compatible with input volumes of up to 10 µg. The purified and size selected DNA is of the highest quality and can then be used in subsequent PCR reactions for next generation sequencing library preparation. The protocol can be completed in approximately 25 minutes.

Kit Specifications
Column Binding Capacity
10 μg of DNA
Maximum Column Loading Volume
600 μL
Size of DNA Purified
Approximately 200-400 bp
Time to Complete 10 Purifications
25 minutes
Minimum Elution Volume
30 μL

Storage Conditions and Product Stability

The Size-Select Buffer should be kept tightly sealed and stored upright, away from light at 4°C. All other solutions should be kept tightly sealed and stored at room temperature (15-25°C) for up to 1 year without showing any reduction in performance.